Enzymatic Synthesis, Structural Analysis, and Evaluation of Antibacterial Activity and α-Glucosidase Inhibition of Hesperidin Glycosides

This study was designed to investigate the structure of synthesized hesperidin glycosides (HGs) and evaluate their antibacterial α-glucosidase inhibitory activities. The preliminary HGs confirmed by glucoamylase treatment analyzed on thin layer chromatography (TLC). LC-MS/MS profiles showed important fragments at m/z ratios 345.21 (added glucose rutinose in HG1) 687.28 maltose HG2), confirming that structures HG1 HG2 were α-glucosyl α-maltosyl hesperidin, respectively. In addition, 1H 13C-NMR derivatives performed identify α-1,4-glycosidic bonds. MIC MBC studies transglycosylated had better bactericidal activities than diosmin, more active against Staphylococcus aureus Escherichia coli. Hesperidin, HG1, HG2, diosmin inhibited with IC50 values 2.75 ± 1.57, 2.48 1.61, 2.36 1.48, 2.99 1.23 mg/mL, inhibition kinetics shown a Lineweaver–Burk plot an competitive inhibitor constant, Ki, 2.20 0.10 mM. Thus, have potential be developed into drugs treatments for treating α-glucosidase-associated type 2 diabetes.